Catalog No. 2501

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Price From: $125.00

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3'...TGCCT (N) 9 ...5'

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Price From: $125.00



Purified from E coli strain that carries the cloned tspGWRI gene from Thermus species GW

Unit Definition

One unit is the amount of enzyme required to digest 1 µg of pBR322 DNA to obtain a stable digestion pattern in 1 hour in a total reaction volume of 50 µl

Reaction Temperature


Heat Inactivation


Assay Unit Substrate

pBR322 DNA

Reaction Buffer

10 mM Tris-HCl (pH 8.5 at 25°C)
1 mM dithiothreitol
10 mM MgCl2 
+Enhancers (1) 


  1. Avoid multiple cycles of freezing / thawing of the stock reaction buffer; No more than three [3] times.
  2. Thawing should be preformed at temperatures not exceeding 10°C.
  3. Recommended procedure is to divide the provided reaction buffer into smaller portions and preserve them at -70°C for long term storage.
  4. Recommended short term storage of reaction buffer is at -20°C.

Storage Buffer

10 mM Tris-HCl (pH 7.5 at 25°C)
0.1 mM EDTA
50 mM NaCl
1 mM dithiothrietol
100 µg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions 

10 mM Tris-HCl (pH 8.5 at 25°C)
10 mM MgCl2 
1 mM dithiothreitol
1 µg pBR322 DNA 
Incubation is at 70°C for 1 hour in a total reaction volume of 50 µl


  1. It is not recommended to use more than 2 units per 50 µl reaction.
  2. Digestion should be performed for over two [2] hours.
  3. As TspGW I binds DNA very tightly, excess amount of TspGW I added can retard DNA migration on a gel, even in the presence of denaturing agents.
  4. TspGW I is very highly stimulated by the presence of two restriction sites in opposite orientation; Both the distance between recognition sequences and their immediate neighborhood also affects the cleavage effectiveness; Single site substrates are cleaved slowly.

Storage Conditions

Store at -20°C
Shipped on dry ice


(1) Zylicz-Stachula, A., Harasimowicz-Slowinska, R., Sobolewski, I. and Skowron, P.M. (2002) Nucleic Acids Research 30, 7 e 33


Certificate of Analysis PDF - Current Lot


Restriction Endonucleases