Tth111 I

Catalog No. 2420

Availability: In stock

Price From: $80.00

Quick Overview

5'...GACN↓NNGTC...3'
3'...CTGNN↑NCAG...5' 

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Price From: $80.00

Details

Source

Thermus thermophilus 111

Reagents Supplied

CMX Buffer 1

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus 2 DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

N/A

Reaction Temperature

65°C

Heat Inactivation

No

Assay Unit Substrate

Adenovirus 2 DNA

Reaction Buffer

10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2 
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Storage Buffer

10 mM Tris-HCl (pH 7.5 at 4°C)
200 mM KCl
0.1 mM EDTA
1 mM dithiothreitol
200 µg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions 

10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg Adenovirus 2 DNA 
Incubation is at 65°C for 1 hour in a total reaction volume of 50 µl

Storage Conditions

Store at -20°C
Shipped on dry ice 

Downloads

MSDS PDF - Current Lot

Categories

Restriction Endonucleases