Alkaline Phosphatase, Calf Intestinal

Catalog No. 1025

Quick Overview

Calf intestinal derived phosphatase (CIP) which catalyzes the hydrolysis of phosphate monoesters

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Price From: $55.00

Details

Source

Calf intestinal mucosa

Reagents Supplied

10X CIP Reaction Buffer

Applications

  • Features higher turnover rate and milder conditions of inactivation than bacterial enzyme (1)
  • Can be used to remove 5'-phosphates from DNA or RNA prior to 5'-end labeling (1)
  • Used to remove 5'-phosphates from linearized vector molecules to prevent self-ligation of the vector during cloning procedures (1)
  • Applicable to dephosphorylation of proteins

Unit Definition

One unit is the amount of enzyme required to hydrolyze 1 µmol of p-nitrophenylphosphate to p-nitrophenol in 1 minute at 37°C (2)

Heat Inactivation

No

Concentration

20 - 25 units/μl

Storage Buffer

10 mM Tris-HCl (pH 8.0)
50 mM KCl
1 mM MgCl2 
0.1 mM ZnCl2 
50% (v/v) glycerol

Assay Conditions

See Certificate of Analysis Below 

Storage Conditions

Store at -20°C
Product shipped on dry ice

Downloads

Certificate of Analysis
Lot 2011002 PDF

MSDS
MSDS PDF 

References

(1) Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition, pp. 5.72, Cold Spring Harbor, New York, NY
(2) Mosser, E., Boll, M., and Pfleiderer, G. (1980) Hoppe-Seyler's Z. Physiol. Chem. 361, 543-549