Polar Alkaline Phosphatase (BAP)

Catalog No. 1027

Availability: In stock

Price From: $55.00

Quick Overview

Thermosensitive Bacterial Alkaline Phosphatase catalyzes the release of 5'- and 3'-phosphate groups from DNA, RNA, and nucleotides.

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Price From: $55.00

Details

Description

  • Removes 5´ phosphates from DNA, RNA, rNTPs and dNTPs.
  • Heat inactivation in 5 minutes at 70°C.
  • Resistant to chemical changes and active over a broad range of buffer conditions.
  • Degradation of dNTPs prior to sequencing of PCR product.
  • Can be used to remove 5'-phosphates from DNA or RNA prior to 5'-end labeling
  • Removes 5'-phosphates from linearized vector molecules to prevent selfligation of the vector during cloning procedures

Applications

  • Ideal for diagnostic immunoassays 
  • Immunodetection of proteins and nucleic acids following blotting experiments
  • Protein dephosphorylation. 

Unit Definition

One unit is the amount of enzyme required to dephosphorylate 1 µg of linearized pUC19 vector DNA in 30 min at 37°C.

Reaction Conditions

1-5 µg of DNA cut with any restriction enzyme
5 µl 10 x Polar-BAP Reaction Buffer
1 µl of Polar-BAP
5 U/μl @ 50 µl
H2O, nuclease free

Storage Buffer

10 mM Tris-HCl (pH 7.6 at 22°C)
1 mM MgCl2
0.01 mM ZnCl2
1 mM DTT
50% glycerol.

1x Polar-BAP Reaction Buffer

50 mM Bis-Tris-Propane-HCl pH 6 @ 25°C
1 mM MgCl2
0.1 mM ZnCl2

Quality Control

All preparations are assayed for contaminating endonuclease and nonspecific RNase and single- and double-stranded DNase activities.

Storage Conditions

Store at -20ºC
Product shipped on dry ice