Poly(A) Polymerase, E. coli

Catalog No. 1240

Availability: In stock

Price From: $55.00

Quick Overview

E. coli derived polymerase, polyadenylating 3' termini of RNA

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Price From: $55.00



Escherichia coli


  • Adds poly A tails to the 3’-hydroxyl terminus of RNA (1)
  • Labels 3’-ends of RNA with a [α-32P] cordycepin triphosphate (2)
  • Ideal for determining molar concentration of polyadenylated RNA (3)

Unit Definition

One unit is the amount of enzyme required to incorporate 1.0 nmol of labeled ATP into acid insoluble form for 10 minutes at 37°C under the stated assay conditions


1 Unit/µl

Storage Buffer

25 mM Tris-HCl (pH 7.9) 
0.1 mM DTT
0.01% (v/v) Triton X-100™
1.0 mM EDTA
0.5 M NaCl50% (v/v) glycerol

Assay Conditions

50 mM Tris-HCl (pH 7.9)
10 mM MgCl2
2.5 mM MnCl
250 mM NaCl
25 µg tRNA
0.1 mM [α-32P] ATP
50 μg bovine serum albumin
Reaction volume of 100 µl

Storage Conditions

Store at -20°C
Product shipped on dry ice


Certificate of Analysis PDF -Current Lot


(1) Sippel, A.E. (1973) Eur. J. Biochem. 37, 31-40
(2) Beltz, W.R. and Ashton, S.H. (1982) Fed. Proc. 41, 1450, Abstract 68996
(3) Krug, M.S., and Berger, S.L. (1986) Anal. Biochem. 153, 315-323