HIV Reverse Transcriptase

Catalog No. 1373

Availability: In stock

Price From: $200.00

Quick Overview

Provides an excellent target for evaluating antiviral agents or inhibitors

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Price From: $200.00

Details

Description

  • Human Immunodeficiency Virus (HIV) Reverse Transcriptase is an RNA directed DNA polymerase which can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template.

Applications

  • Provides an excellent target for evaluating antiviral agents or inhibitors (1,2)
  • Catalyzes error-prone synthesis on DNA and RNA templates - ideal for introducing random mutations (3)

Unit Definition

One unit is the amount of enzyme required to incorporate 1 nmol of labeled dTTP into acid insoluble material in 10 minutes
at 37°C.

Concentration

10 - 30 units/µl

Assay Conditions

50 mM Tris-HCl (pH 8.3)
6 mM MgCl2
40 mM KCl
0.5 mM [3H] TTP
0.4 mM poly rA:dT
Reaction volume of 50 µl

Storage Buffer

20 mM potassium phosphate (pH 7.1)
1.0 mM dithiothreitol
0.02% Triton X-100™
50% (v/v) glycerol

Reaction Buffer

10X HIV-RT Reaction Buffer

Storage Conditions

Store at -20°C
Product shipped on Dry Ice

Downloads

MSDS PDF

References

1. Hirsch, M. S., Kaplan, J. C. (1985) Ann. Intern. Med. 103, 750-755
2. Tisdale, M. et al (1989) J. Antimicrob. Chemother. 23, 47-54
3. Williams, K. J., Loeb, L. A. (1992) Curr. Top. Microbiol. Immunol. 176, 80-165