Thermophilic Pyrophosphatase

Catalog No. 1267

Quick Overview

Improves PCR amplification of problematic of long templates

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Details

Source

Thermus aquatieus

Applicatons

  • Hydrolyzes pyrophosphate, a common product of biosynthetic reacions (1)
  • Maintains the forward direction of reactions generating pyrophosphate
  • Improves PCR amplification of problematic of long templates
  • Used as an enzyme label of ELISA (2)

Unit Definition

One unit will liberate 1.0 nmol of inorganic orthophosphate per minute at 65°C (pH 7.5)

Storage Buffer

20 mM Tris-HCl (pH 8.0 at 4°C)
50 mM imidazole
100 mM KCl
0.1 mM EDTA
50% (v/v) glycerol
0.5% Triton X-100

Assay Conditions

40 mM Tris-HCl (pH 7.5 at 22°C)
40 mM sodium acetate
40 mM imidazole
1.0 mM sodium pyrophosphate.
2.5 mM MgCl2
Reaction volume 100 μl

Quality Control

All preparations are assayed for contaminating endonuclease, exonuclease and nonspecific RNase and single- and double-stranded DNase activites.

Storage Conditions

Store at -80°C
Shipped on Dry Ice

Downloads

MSDS PDF -Current Lot

References

Baykov, A.A., Kasho, V.N. and Avaeva, S.M. (1988) Anal. Biochem. 171, 271-276.2. Jerhoeven, J.A. (1986) J. Bacteriol. 168, 318-321
Richter, O.M. and Schafer, G. (1992) Eur. J. Biochem. 209, 343-349