HeLa Extract

Catalog No. 6030

Quick Overview

HeLa cytoplasmic extract

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$1,200.00

Details

Source

Cultured HeLa Cells

Function Definition

HeLa Extract is required to show SV40 DNA replication in the presence of 1 µg SV40 Large T Antigen in vitro (1)

Storage Buffer

20 mM Hepes (ph 7.5)
50 mM NaCl
1 mM dithiothreitol
0.1 mM EDTA
10% (v/v) glycerol

Assay Conditions 

According to the method of Li and Kelly, as provided in the SV40 DNA Replication Assay, CHIMERx catalog number 8050 (1), (2), (3)

Storage Conditions

Store at -80ºC
Once thawed, aliquots of HeLa Extract that are not used should be discarded
Freeze-thaw cycles will result in diminished performance of the extract

References 

(1) SV40 DNA Replication Assay Kit, CHIMERx Catalog number 8050, Instruction Manual, Version 1.6
(2) Li, Joachim J. and Kelly, Thomas J. (1985) Mol Cell Biol 5, 1238-1246
(3) Stillman, B.W. and Gluzman, Y. (1985) Mol Cell Biol 5, 2051-2060

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF - Current Lot 

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