OptiTaq DNA Polymerase

Catalog No. 1102

Quick Overview

Mixture of stable thermophilic DNA polymerases capable of generating PCR products up to 20 kb with high fidelity; suitable for applications requiring high temperature synthesis of DNA.

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Price From: $160.00

Details

Description

  • OptiTaq DNA Polymerase is a modified and optimized thermostable enzymes blend containing Thermus aquaticus DNA polymerase and Pyrococcus sp. DNA polymerase.
  • Ultrapure, recombinant enzymes are used to prepare OptiTaq DNA Polymerase.
  • The enzymes blend exhibits the 3'→5' proofreading activity, resulting in considerably higher PCR fidelity and processivity than possible with unmodified Taq DNA polymerase (1).
  • Enables increased amplification product yield in comparasion with Taq DNA polymerase over wide range of PCR products.
  • Maintains the 5'→3'  exonuclease activity.
  • Adds extra A and the 3' ends.
  • Suitable for multiplex PCR as it exhibits wider tolerance for Mg2+, salts concentration and pH (2,3).
  • Improves PCR results with critical templates, such as containing GC-rich regions, palindromes or multiple repeats.
  • OptiTaq DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 20 kb.

Storage Buffer

20 mM Tris-HCI (pH 8.0 at 22°C)
100 mM KCI
0.5% Tween™20
0.5% Igepal CA-630
0.1 mM EDTA
1 mM dithiothreitol
50% glycerol

10X Reaction Buffers

10X Pol Buffer A (optimization buffer without MgCl2):
The buffer allows to optimize MgCl2 concentration.

10X Pol Bufer B (general application, up to 20 kb):
The buffer contains 15 mM MgCl2 and is optimized for use with 0.2 mM of each dNTP.

10X Pol Buffer C (colored):
10X Pol buffer B enriched with two gel tracking dyes and a gel loading reagent. The buffer enables direct loading of PCR products onto an agarose gel.

Quality Control

All preparations are assayed for contaminating endonuclease, 3'-exonuclease and nonspecific single- and double-stranded DNase activities. Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis.

References

(1) Cline, J., Braham, J. and Hogrefe, H. (1996) Nucleic Acids Res. 24, 3546.
(2) Chien, A., Edgar, D.B. and Trela, J.M. (1976) J. Bacteriol. 127, 1550.
(3) Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980) Biokhimiya 45, 644.