pUC19 Vector

Applications

• Cloning
• Sequencing
• Manufacturing DNA Size Markers

Vector Identity

Identity and homogeneity of pUC19 Vector were confirmed by a series of restriction enzyme digests followed by analysis of the resulting DNA fragment pattern.

Storage Buffer

10 mM Tris-HCl (pH 7.8)
1 mM EDTA

Storage Conditions 

Store at 4°C short term (2 weeks or less)
Store at -80°C long term (longer than 2 weeks)
Avoid multiple freeze-thaw cycles 

Notes

• pUC19 Vector manufactured by CHIMERx is ≥90% supercoiled
• pUC19 Vector is ampicillin resistant
• The multiple cloning site contains recognition sequences for the following restriction enzymes: Acc I, Acc651, Apo I, Ava I, BamH I, Ban II, BfuA I, BsoB I, BspM I, Eco53K I, EcoR I, Hinc II, Hind III, Kpn I, Pst I, Sac II, Sal I, Sbf I, Sma I, Sph I, TspM I, Xba I and Xma I