SV40 Large T Antigen

Catalog No. 5800

Quick Overview

Exclusively from CHIMERx, simian virus (SV40) DNA is the best model for mammalian chromosomal DNA replication in vitro

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Details

Source

Simian virus 40

Description

The major regulatory protein of SV40 virus, responsible for virion assembly, viral and cellular transcriptional regulation, viral DNA replication, and alteration of the cell cycle

SV40 Large T Antigen plays multifunctional roles:

  • Functions as an initiator protein to begin DNA replication at the SV40 origin
  • Performs as a DNA helicase that unwinds DNA ahead of the polymerizing complex
  • Acts as a primosome loading protein
  • Exhibits ATPase activity

Applications

  • Simian virus (SV40) DNA is the best model for mammalian chromosomal DNA replication in vitro (1)
  • Examining fidelity and biochemistry of DNA replication in humans (6,7)
  • Identifying mode of action and mechanisms leading to cell transformation in vitro and tumor formation in vivo (8)
  • Regulating gene expression in eukaryotic cells (2)
  • Studying the inhibition of DNA replication by mutagenesis (3,4)
  • Determining risks posed by human carcinogens
  • Comparing replication of normal and malignant cells
  • Investigating the cytotoxic effects of drugs (5)
  • Investigating apoptosis

Quality Control

All preparations are assayed for stimulation of DNA synthesis in a T antigen-dependent DNA replication assay (2)
Typical preparations are greater than 90% pure as judged by SDS polyacrylamide gel electrophoresis

Storage Conditions

Store at -20°C
Shipped on Dry Ice

Note

This product is functionally waranted only when used in conjunction with our SV40 DNA Replication Assay Kit, Catalog Number 8050, containing control DNA, reaction buffer, and HeLa Extract

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF 

References

1. Evan, G. and Littlewood, T. (1998) Science 281,1317-1321
2. Wold, M.S., Weinberg, D.H., Virshup, D.M., Li, J.J. and Kelly, T.J. (1989 ) J. Biol. Chem. 264, 2801-2809
3. Thomas, D.C., Veaute, X., Kunkel, T.A. and Fuchs, R.P.P. (1994) Proc. Natl. Acad. Sci. 91, 7752-7756
4. Boyer, J.C., Thomas, D.C., Maher, V.M., McCormick, J.J. and Kunkel, T.A. (1993) Cancer Res. 53, 3270-3275
5. Bebenek, K., Thomas, D.C., Roberts, J.D., Eckstein, F. and Kunkel, T.A. (1993) Mol. Pharm. 43, 57-63
6. Stillman, B. (1994) Cell 78, 725-728
7. Fanning, E. (1992) J. Virol. 66, 1289-1293
8. Dixon, R.A.F., and Nathans, D. (1985) J. Virol. 53, 1001-1004