T4 Gene 32 Protein

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Description

• Single-stranded specific DNA binding protein (1)
• Helix destabilizing protein (1)
• Ultrapure recombinant protein

Applications

• Reduces formation of secondary DNA structures
• Prevents degradation of ssDNA by nucleases
• Prevents inhibition of PCR by template NDA contaminants (2)
• Replaces Hot Start method during assembling PCR reaction (4,7)
• Improves the efficiency of DNA amplification by Taq Polymerase (3,4,5,6)
• Enchances the fidelity of modified T4 Polymerase (8)
• Stabilizes single-stranded regions of DNA for site-specific mutagenesis
• Aids completion of restriction enzyme digestion
• Improves the efficiency of DNA synthesis by T4 DNA Polymerase (8)

Quality Control

All preparations are assayed for contaminating endonuclease, 3'- and 5'-exonulease activities. Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis

Storage Buffer

20 mM Tris-HCl (pH 8.0 at 22°C)
100 mM NaCl
1 mM DTT
1 mM EDTA
100 mM NaCl
50% (v/v) glycerol 

Storage Conditions

Store at -20°C
Shipped on Dry Ice

References

(1) Greipel, J. Urbanke, C. and Maass, G. (1989) in: Saenger, W., Heinemann, U. (Eds.) pp.61-86
(2) Kreader, C.A. (1996) Applied Environ. Micro. 62, 1102-1106
(3) Dabrowski, S., Olszewski, M., Piatek, R. and Kur, J. (2002) Protein Expr. Purif. 26, 131-138
(4) Dabrowski, S. and Kur, J. (1999)Protein Expr. Purif. 16, 96-102
(5) Rapley, Mol. Biotech. 2 (1994) 295-298
(6) Schwarz, K., Hansen-Hagge, T. and Bartram, C. (1989) Nucleic Acids Res. 18, 1079
(7) Barski, P., Piechowicz, L., Galinski, K. and Kur, J. (1996) Mol. Cell Probes 10, 471-475
(8) Sandhu, D.K. and Keohavong, P. (1994) Gene 144, 53-58