Apa l

Source

Acetobacter pasteurianus

Reagents Supplied

CMX Buffer 4

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus 2 DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

No

Incubation Temperature

30°C

Heat Inactivation

65°C for 10 minutes

Assay Unit Substrate

Adenovirus 2 DNA

Reaction Buffer

CMX Buffer 4:
20 mM Tris-acetate (pH 7.5 at 37°C)
10 mM magnesium acetate
50 mM potassium acetate
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 100%
Buffer 2: 75%
Buffer 3: 0%
Buffer 4: 100%
Buffer 5: 75%

Storage Buffer

50 mM Tris-HCl (pH 7.5 at 22°C)
50 mM KCl
1 mM EDTA
10 mM BME
0.1% Triton X-100™
200 µg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions 

See Certificate of Analysis Below

Storage Conditions 

Store at -20°C
Shipped on dry ice