BstX I

Source

Bacillus stearothermophilus X

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour at 55°C in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

55°C

Assay Unit Substrate

Lambda DNA

Heat Inactivation

65°C for 10 minutes

Reaction Buffer

CMX Buffer 3:
50 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂
100 mM NaCl
1 mM DTT
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: <25%
Buffer 2: 100%
Buffer 3: 100%
Buffer 4: 50%
Buffer 5: 50%
*star activity 

Assay Conditions

See Certificate of Analysis Below

Storage Buffer

10 mM Tris‑HCl (pH 7.5 at 4°C)
50 mM KCl
0.1 mM EDTA
1 mM dithiothreitol
0.005% Triton X-100™
200 µg/ml bovine serum albumin
50% (v/v) glycerol

Conditions

Store at -20°C
Shipped on dry ice