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Cycle-Sequal™ DNA Polymerase
Cycle-Sequal™ DNA Polymerase. Stable thermophilic DNA polymerase suitable for applications requiring high-temperature synthesis of DNA.
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SKU
8020
Description
Cycle-Sequal™ DNA Polymerase is a stable thermophilic DNA polymerase suitable for applications requiring high temprature synthesis of DNA
Unit Definition
One unit is the amount of enzyme required to incorporate 10 nmoles of total nucleotide into acid-insoluble material in 30 minutes at 37°C
Assay Conditions
25 mM Tris-HCl (pH 9.5)
10 mM MgCl2
1.0 mg/ml of bovine serum albumin
50 mM KCl
0.2 mM of each dCTP, dGTP and dTTP
15 µg activated DNA
Storage Buffer
50 mM Tris HCl (pH 7.5)
5.0 mM DTT
0.1 mM EDTA
50% (v/v) glycerol
Storage Conditions
Store at -20°C
Product shipped on dry ice
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