Mung Bean Nuclease

Mung Bean Nuclease Source

Mung bean sprouts

Reagents Supplied

10X Mung Bean Nuclease Reaction Buffer

Applications

• Removes protruding ends in duplex DNA resulting in ligatable blunt ends (1).
• Suitable for trimming single-stranded protruding ends of DNA or RNA without degrading the duplex structure (2).
• Used for mapping transcripts by analyzing the Mung Bean Nuclease-resistant RNA-DNA hybrids (3).
• Digests hairpin structures during cDNA synthesis (4).
• Will not cleave the strand opposite a nick in duplex DNA.
• Requires Zn²⁺ ions for activity.

Unit Definition

One unit is the amount of enzyme required to produce 1 µg of acid-soluble material per minute at 37°C using denatured calf thymus DNA.

Reaction Buffer

30 mM NaOAc (pH 5.0 at 22°C)
50 mM NaCl
1 mM ZnCl₂ 
5% glycerol 

Storage Buffer

10 mM Tris-HCl (pH 7.5 at 22°C)
0.1 mM zinc acetate
50% (v/v) glycerol
1 mM Cystein 

Assay Conditions 

30 mM sodium acetate (pH 5.0 at 22°C).
50 mM NaCl.
1 mM ZnCl_2.
0.5 mg/ml denatured calf thymus DNA.
5% (v/v) glycerol.
Incubation at 37ºC for 10 minutes in a total reaction volume of 1 ml.

Quality Control

All preparations are assayed for contaminating double-stranded DNase activity.