Sst II

Source

Streptomyces achromogenes

Unit Definition

One unit is the amount of enzyme required to completely digest     1 µg of Adenovirus 2 DNA in 1 hour in a total reaction volume of 50 µl.

Reaction Temperature

37°C

Heat Inactivation

20 minutes at 65°C

Reaction Buffer

10 mM Tris-HCl (pH 7.5 at 22°C)
10 mM MgCl₂
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Storage Buffer

20 mM Tris‑HCl (pH 8.0)
150 mM KCl
500 µg/ml bovine serum albumin
0.1% Triton X-100™
50% (v/v) glycerol

Assay Conditions

50 mM Tris‑HCl (pH 8.0)
10 mM MgCl₂
50 mM NaCl
1 µg Adenovirus 2 DNA
Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl.

Sst II Storage Conditions

Store at -20°C
Shipped on dry ice