Human DNA Polymerase Gamma

Description

• Mitochondrial enzyme used for testing the toxicity of drugs on critical human oxidative functions and testing replication of mitochondrial DNA

Unit Definition

One unit is the amount of enzyme required to incorporate 1 pmole of total nucleotide into acid-insoluble form in 60 minutes at 37°C

Storage Buffer

20 mM Tris-HCl (pH 8.0)
50 mM NaCl
50 µg/ml PMSF
0.05% Triton X-100™
10% DMSO
5% glycerol

Assay Conditions

25 mM Hepes-KOH (pH 8.0)
0.5 mM MnCl₂
10 µg bovine serum albumin
2.5 mM β-mercaptoethanol
100 mM NaCl
10 µM TTP and 0.3 µCi [3H]TTP
0.29 mM poly rA:(dT)50
Incubation for 15 minutes at 37°C in a total reaction volume of 15 μl

Quality Control

All preparations are assayed for contaminating endonuclease and exonuclease activities

Storage Conditions

Store at -80°C Long Term, -20°C Short Term
Avoid multiple freeze / thaw cycles
Shipped on dry ice