S1 Nuclease

Source

Aspergillus oryzae

Applications

• Catalyzes degradation of single-stranded nucleic acids into oligonucleotides and 5'-mononucleotides (1)
• Cleaves both single-stranded DNA and RNA, with stronger DNAse activity
• Double-stranded DNA, double-stranded RNA and DNA-RNA hybrids are resistant to degradation at moderate enzyme concentration
• Capable of cleavage of double-stranded nucleic acids at nicks, mismatches and small gaps (2)
• Relaxes/linearizes supercoiled plasmids
• Removes protruding single-stranded ends
• Can generate double-stranded DNA breaks in response to DNA nicks abasic sites
• Used for S1 mapping of nucleic acids
• Requires Zn²+ ions for activity
• The enzyme is active up to 65°C

Unit Definition

One unit is the amount of enzyme required to convert 1 µg of denatured calf thymus DNA to an acid soluble form in 1 minute at 37°C.

Storage Buffer

20 mM Sodium Acetate (pH 4.6)
50 mM NaCl
1 mM ZnCl₂
50% (v/v) Glycerol

Quality Control

All preparations are assayed for contaminating exonuclease and double-stranded DNase activities.

Storage Conditions

Store at -20°C
Product shipped on Dry Ice