Dra l

Source

Deinococcus radiophilus

Reagents Supplied

CMX Buffer 4

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

No

Incubation Temperature

37°C

Heat Inactivation

65°C for 10 minutes

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 4:
20 mM Tris-Acetate (pH 7.5 at 37°C)
50 mM potassium acetate
10 mM magnesium acetate
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 75%
Buffer 2: 100%
Buffer 3: 100%
Buffer 4: 100%
Buffer 5: 0%

Storage Buffer

50 mM Tris‑HCl (pH 7.4 at 4°C)
50 mM NaCl
0.1 mM EDTA
1 mM dithiothreitol
500 µg/ml bovine serum albumin
0.1 mM phenylmethylsulfonyl fluoride
50% (v/v) glycerol

Assay Conditions

See Certificate of Analysis Below

Storage Conditions

Store at -20°C
Shipped on dry ice