EcoR I

Source

Escherichia coli RY 13

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

65°C for 10 minutes

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 3:
50 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂
100 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 50%
Buffer 2: 100%
Buffer 3: 100%
Buffer 4: 75%
Buffer 5: 75%
*Star activity 

Storage Buffer

50 mM Tris-HCl (pH 7.2 at 4°C)
300 mM NaCl
0.5 mM EDTA
5 mM EGTA
0.2% (v/v) Triton X-100™
5 mM BME
5 µg/ml PLL
500 µg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions

See Certificate of Analysis Below

Storage Conditions

Store at -20°C
Shipped on dry ice