Hinf I

Source

Haemophilus influenzae Rf

Reagents Supplied

CMX Buffer 2

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour at 37°C in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

80°C for 10 minutes

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 2:
10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 25%
Buffer 2: 100%
Buffer 3: 75%
Buffer 4: 75%
Buffer 5: 50%
*Star activated 

Storage Buffer

50 mM Tris-HCl (pH 7.5 at 4°C)
0.1 mM EDTA
10 mM β-mercaptoethanol
500 µg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions

10 mM Tris‑HCl (pH 7.5 at 37°C)
10 mM MgCl₂
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg lambda DNA
Reaction volume of 50 µl

Storage Conditions

Store at -20°C
Shipped on dry ice