Hpa II

Source

Haemophilus parainfluenzae

Reagents Supplied

CMX Buffer 1

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

No

Incubation Temprature

37°C

Heat Inactivation

65°C for 10 minutes

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 1:
10 mM Tris-HCl (pH 7.0 at 37°C)
10 mM MgCl₂
1 mM dithiothreitol

Activity in Buffers

Buffer 1: 100%
Buffer 2: 75%
Buffer 3: 25%
Buffer 4: 75%
Buffer 5: 100%

Storage Buffer

20 mM Tris-HCl (pH 7.4 at 4°C)
0.5 mM EDTA
1 mM dithiothreitol
0.5 mg/ml bovine serum albumin 
50% (v/v) glycerol

Assay Conditions

10 mM Tris-HCl (pH 7.0 at 37°C)
10 mM MgCl₂
1 mM dithiothreitol
100 μg/ml bovine serum albumin 
1 μg lambda DNA
Incubation is at 37°C for 1 hour in a reaction volume of 50 µl

Storage Conditions

Store at -20°C
Shipped on dry ice