Kpn I

Source

Klebsiella pneumoniae OK 8

Reagents Supplied

CMX Buffer 1

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus 2 DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

No

Assay Unit Substrate

Lambda/Hind III DNA fragments

Reaction Buffer

CMX Buffer 1:
10 mM Tris-HCl (pH 7.0 at 37°C)
10 mM MgCl₂
1 mM dithiothreitol

Activity in Buffers

Buffer 1: 100%
Buffer 2: 50%
Buffer 3: NA
Buffer 4: 75%
Buffer 5: 50%
Buffer 6: <25%
*Star activity 

Storage Buffer

10 mM Tris-HCl (pH 7.4 at 4°C)
0.1 mM EDTA
200 mM KCl
1 mM DTT
0.05% (v/v) TWEEN-20™
500 μg/ml bovine serum albumin
50% (w/v) glycerol

Assay Conditions

10 mM Tris‑HCl (pH 7.0 at 37°C)
10 mM MgCl₂
1 mM dithiothreitol
1 µg Lambda/Hind III DNA fragments

Storage Conditions

Store at -20°C
Shipped on dry ice