OptiTaq DNA Polymerase

Description

• OptiTaq DNA Polymerase is a modified and optimized thermostable enzymes blend containing Thermus aquaticus DNA polymerase and Pyrococcus sp. DNA polymerase.
• Ultrapure, recombinant enzymes are used to prepare OptiTaq DNA Polymerase.
• The enzymes blend exhibits the 3'→5' proofreading activity, resulting in considerably higher PCR fidelity and processivity than possible with unmodified Taq DNA polymerase (1).
• Enables increased amplification product yield in comparasion with Taq DNA polymerase over wide range of PCR products.
• Maintains the 5'→3'  exonuclease activity.
• Adds extra A and the 3' ends.
• Suitable for multiplex PCR as it exhibits wider tolerance for Mg²⁺, salts concentration and pH (2,3).
• Improves PCR results with critical templates, such as containing GC-rich regions, palindromes or multiple repeats.
• OptiTaq DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 20 kb.

Storage Buffer

20 mM Tris-HCI (pH 8.0 at 22°C)
100 mM KCI
0.5% Tween™20
0.5% Igepal CA-630
0.1 mM EDTA
1 mM dithiothreitol
50% glycerol

10X Reaction Buffers

10X Pol Buffer A (optimization buffer without MgCl₂):
The buffer allows to optimize MgCl₂ concentration.

10X Pol Bufer B (general application, up to 20 kb):
The buffer contains 15 mM MgCl₂ and is optimized for use with 0.2 mM of each dNTP.

10X Pol Buffer C (colored):
10X Pol buffer B enriched with two gel tracking dyes and a gel loading reagent. The buffer enables direct loading of PCR products onto an agarose gel.

Quality Control

All preparations are assayed for contaminating endonuclease, 3'-exonuclease and nonspecific single- and double-stranded DNase activities. Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis.