PinA I

PinA I Source

Pseudomonas inaequalis

Reagents Supplied

CMX Buffer 5

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in total reaction volume of 50 µl

Star Activity

No

Incubation Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 5:
20 mM Tris-HCl (pH 7.0 at 37°C)
5 mM MgCl₂
50 mM KCl
1 mM dithiothreitol
0.1 mg/ml bovine serum albumin 

Activity in Buffers

Buffer 1: 50%
Buffer 2: 75%
Buffer 3: 50%
Buffer 4: 50%
Buffer 5: 100%

Storage Buffer

20 mM Tris-HCl (pH 7.5 at 4°C)
0.1 mM EDTA
100 mM NaCl
10 mM β-mercaptoethanol
0.05% (v/v) Triton X-100™
50% (v/v) glycerol

Assay Conditions

20 mM Tris‑HCl (pH 7.0 at 37°C)
5 mM MgCl₂
50 mM KCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg lambda DNA
Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl

Storage Conditions

Store at -20°C
Shipped on dry ice 

Categories

Restriction Endonucleases