Human DNA Polymerase Beta

Description

• Simplest DNA polymerase known in both size and catalysis (1)

Applications

• Repair polymerase able to synthesize DNA beyond the end of a gap or nick with simultaneous displacement of the non-replicated strand (2)
• Fills gaps and nicks (3)

Unit Definition

One unit is the amount of enzyme required to incorporate 1 pmole of total nucleotide into acid-insoluble form in 60 minutes at 37°C under the stated assay conditions

Storage Buffer

20 mM Tris-HCl (pH 8.0)
0.1 mM EDTA
200 mM NaCl
1 mM dithiothreitol
50% (v/v) glycerol

Assay Conditions

50 mM Tris-HCl (pH 8.7)
100 mM KCl
10 mM MgCl₂
0.4 mg/ml bovine serum albumin
1.0 mM dithiothreitol
15% (v/v) glycerol
0.05 mM of each dCTP, dGTP, dTTP, and [α-³²P]dATP
10 μg activated DNA
Reaction volume of 50 μl

Quality Control

All preparations are assayed for contaminating endonuclease and exonuclease activities

Storage Conditions

Store at -80°C Long Term
Store at -20°C
Product shipped on dry ice