AMV Reverse Transcriptase in Trehalose

Source

Avian Myeloblastosis Virus

Description

• Contains both reverse transcriptase and RNase H activities
• Maintains RNase H activity necessary for cDNA synthesis and isothermal RNA amplification
• Can be used for preparing labeled hybridization probes
• Synthesizes cDNA from single-stranded RNA
• Ideal for use in RT-PCR, RAMP™, NASBA™, cDNA libraries and dideoxy-DNA sequencing (1, 2, 3)

Reagents Supplied

5X AMV Reverse Transcriptase Reaction Buffer

Unit Definition

One unit is the amount of enzyme required to incorporate 1.0 nmol of labeled dTTP into acid insoluble material in 10 minutes at 37°C (4)

Concentration

15-30 units/µl

Storage Buffer

200 mM potassium phosphate (pH 7.1)
2 mM dithiothreitol
1M Trehalose

Assay Conditions

50 mM Tris‑HCl (pH 8.3)
10 mM MgCl₂
40 mM KCl
0.2 mM poly rA:dT
[³H] dTTP
Reaction volume is 100µl

Storage Conditions

Store @ -80°C in a Non-frost-free Freezer
Shipped on Dry Ice