HIV Reverse Transcriptase

Description

• Human Immunodeficiency Virus (HIV) Reverse Transcriptase is an RNA directed DNA polymerase which can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template.

Applications

• Provides an excellent target for evaluating antiviral agents or inhibitors (1,2)
• Catalyzes error-prone synthesis on DNA and RNA templates - ideal for introducing random mutations (3)

Unit Definition

One unit is the amount of enzyme required to incorporate 1 nmol of labeled dTTP into acid insoluble material in 10 minutes
at 37°C.

Concentration

10 - 30 units/µl

Assay Conditions

50 mM Tris-HCl (pH 8.3)
6 mM MgCl₂
40 mM KCl
0.5 mM [³H] TTP
0.4 mM poly rA:dT
Reaction volume of 50 µl

Storage Buffer

20 mM potassium phosphate (pH 7.1)
1.0 mM dithiothreitol
0.02% Triton X-100™
50% (v/v) glycerol

Reaction Buffer

10X HIV-RT Reaction Buffer

Storage Conditions

Store at -20°C
Product shipped on Dry Ice