Perpetual OptiTaq DNA Polymerase

Description

• Perpetual OptiTaq DNA Polymerase is a modified and balanced blend containing top quality Thermus aquaticus DNA Polymerase, Pyrococcus furiosus DNA Polymerase, anti-Taq DNA Polymerase antibodies, reversible inhibitors and enhancers.
• Ultrapure, recombinant proteins are used to prepare Perpetual OptiTaq DNA Polymerase.
• Our carefully selected anti-Taq antibodies have high thermal stability, providing protection against non-specific primer extension from room temperature to 70°C.
• The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 92-95°C for two minutes.
• Formation of inactive complexes between Taq DNA Polymerase and an anti-Taq antibody forms a basis for automatic "hot start" PCR, which allows for the assembly of PCR reactions at room temperature.
• High stability of the complexes allows for the enormous increase of PCR specificity, sensitivity and yield in comparison to the conventional PCR assembly method.
• Automatic "hot start" PCR is a convenient method when assembling multiple PCR reactions, saving time and reagents.
• Clean and safe laboratory practice assured, due to removed necessity to open hot tubes.
• The blend catalyzes the polymerization of nucleotides into duplex DNA in the 5'→3' direction in the presence of magnesium ions and exhibits the 3'→5' proofreading activity, resulting in considerably higher PCR fidelity than possible with unmodified Taq DNA polymerase (1).
• Enables increased amplification product yield in comparison with Taq DNA polymerase over wide range of PCR products.
• Maintains the 5'→3´ exonuclease activity.
• Adds extra A at the 3' ends.
• Suitable for multiplex PCR due to increased specificity, wider tolerance for Mg²⁺, salts concentration, and pH (2,3).
• Improves PCR results with critical templates, such as containing GC-rich regions, palindromes or multiple repeats.
• Perpetual OptiTaq DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 20 kb.

Storage Buffer

20 mM Tris-HCL (pH 8.0 at 22°C)
100 mM KCI
0.5% Igepal CA-630
0.1 mM EDTA
1 mM dithiothreitol
50% glycerol

10X Reaction Buffers

10X Pol Buffer A (optimization buffer without MgCl₂)
The buffer allows to optimize MgCl₂ concentration.

10X Pol Buffer B (general application without MgCl₂)
The buffer contains 15 mM MgCl₂ and is optimized for use with 0.2 mM of each dNTP.

10X Pol Buffer C (colored)
10X Pol Buffer B enriched with two gel tracking dyes and a gel loading reagent. The buffer enables direct loading of PCR products onto an agarose gel.

Quality Control

All preparations are assayed for contaminating endonuclease, 3'-exonuclease, and nonspecific singe- and double-stranded DNase activities. Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis.