Tth DNA Polymerase

Reagents Supplied

10X Tth DNA Polymerase Reaction Buffer

Source

Thermus thermophilus

Applications

Ccan reverse transcribe at elevated temperatures
Minimizes problems with strong secondary structure of RNA
Results in greater specificity of primer hybridization and extension
Suitable for high temperature synthesis of DNA
Synthesizes cDNA from RNA template
Stable thermophilic DNA polymerase (1)

Unit Definition

One unit is the amount of enzyme required to incorporate 10 nmoles total nucleotide into acid insoluble material at 70°C for 30 minutes.

Concentration

5 - 10 units/µl

Storage Buffer

50 mM Tris HCl (pH 7.5)
0.1 mM EDTA
5.0 mM dithiothreitol
50% (v/v) glycerol
Stabilizers

Assay Conditions

25 mM Tris-HCl (pH 9.5 at 25°C)
50 mM potassium chloride
10 mM magnesium chloride
1 mM dithiothreitol
0.3 µg/μl activated calf thymus DNA
1 μg/μl bovine serum albumin
0.2 mM of each dCTP, dGTP, dTTP and [α-³²P]dATP
Incubation is for 10 minutes at 70°C in a total reaction volume of 50 µl

Storage Conditions

Store at -20°C
Product shipped on dry ice