RecA Protein

Description

• A 37,8 kDa protein encoded by the recA gene of E coli.
• Involved in DNA renaturation and rare cleavage of genomic DNA.
• Ultrapure recombinant protein.

Applications

• Regulates repair mechanisms and repairs DNA (1, 2).
• Binds cooperatively and stechiometrically to single-stranded DNA.
• In presence of ATP-g-S, locates and pairs a single-stranded DNA sequence to its homologous double-stranded DNA.
• Cleaves large fragments of DNA (RARE-Rec A Assisted Restriction Endonuclease) (3).
• Enhances contrast of electron micrography when DNA is coated with Rec A Protein (4).

Storage Buffer

20 mM Tris-HCl (pH 7.5 at 22°C)
1 mM Dithiothreitol
0.1 mM EDTA  
50% (v/v) Glycerol

Quality Control

All preparations are assayed for endonuclease and nonspecific single- and double-stranded DNase activities. Typically preparations are greater than 95% pure as judged by SDS polyacrylamide gel electrophoresis.

Storage Conditions

Store at -20°C
Shipped on Dry Ice