Acc I

Source

Acinetobacter calcoaceticus

Reagents Supplied

CMX Buffer 4

Unit Definition 

One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour in a total reaction volume of 50 μl

Star Activity

No

Incubation Temperature

37°C

Heat Inactivation

10 minutes at 80°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 4:
20 mM Tris-acetate (pH 7.5 at 37°C)
10 mM Magnesium Acetate
50 mM Potassium Acetate
1 mM Dithiothreitol
100 μg/mL Bovine Serum Albumin

Activity in Buffers

Buffer 1: <25%
Buffer 2: >75%
Buffer 3: 25%
Buffer 4: 100%
Buffer 5: 100%

Storage Buffer

50 mM Tris-HCl (pH 7.5 at 4°C)
10 mM BME
0.1 mM EDTA
0.5 mg/ml bovine serum albumin
0.1% Triton X-100™
50% (v/v) glycerol

Storage Conditions

Store at -20°C
Shipped on Dry Ice