Acv I

Source

Aeromonas caviae

Reagents Supplied

CMX Buffer 4

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA/Hind III fragments in 1 hour in a total reaction volume of 50 µl

Star Activity

No

Incubation Temperature

37°C

Heat Inactivation

80° for 10 minutes

Assay Unit Substrate

Ad-2 DNA

Reaction Buffer

CMX Buffer 4:
20 mM Tris-Acetate (pH 7.5 at 37°C)
10 mM Magnesium Acetate
50 mM Potassium Acetate
1 mM Dithiothreitol
100 µg/ml Bovine Serum Albumin

Activity in Buffers

Buffer 1: 75%
Buffer 2: 100%
Buffer 3: 50%
Buffer 4: 100%
Buffer 5: 75%

Storage Buffer

10 mM Tris-HCl (pH 7.5 at 4°C) 
1 mM Dithiothreitol
50 mM KCl
0.1 mM EDTA
10 mM MgCl₂
200 µg/ml Bovine Serum Albumin  
50% (v/v) Glycerol

Assay Conditions

20 mM Tris-Acetate (pH 7.5 at 37°C)
10 mM Mg Acetate
50 mM K Acetate
1 mM Dithiothreitol 
100 µg/ml Bovine Serum Albumin  
1 µg Ad-2 DNA

Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl

Storage Conditions

Store at -20°C
Shipped on Dry Ice