Bal I

Bal I Source

Brevibacterium albidum

Reagents Supplied

CMX Buffer 4

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour in a total reaction volume of 30 μl

Heat Inactivation

65°C for 10 minutes

Incubation Temperature

37°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 4:
20 mM Tris-acetate (pH 7.5 at 37°C)
10 mM magnesium acetate
50 mM potassium acetate
1 mM dithiothreitol
100 μg/ml bovine serum albumin

Storage Buffer

10 mM Tris-HCl (pH 7.5 at 22°C)
1 mM dithiothreitol
50 mM KCl
0.1 mM EDTA
200 μg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions

20 mM Tris-acetate (pH 7.5 at 37°C)
10 mM magnesium acetate
50 mM potassium acetate
1 mM dithiothreitol
100 μg/ml bovine serum albumin
1 μg of lambda DNA
Incubation for 1 hour at 37°C in a total reaction volume of 30 μl

Quality Control

All preparations are assayed for contaminating endonuclease, 3'-exonuclease, and nonspecific single- and double-stranded DNase activities

Storage Conditions

Store at -20°C
Shipped on Dry Ice