BsiHKC I

Source

Bacillus stearothermophilus HKC
Note: Purified from E coli strain that carries the cloned bsiHKCRI gene.

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour in a total reaction volume of 50 μl

Star Activity

Yes

Incubation Temperature

55°C

Heat Inactivation

No

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 3:
50 mM Tris-HCl (pH 7.5 at 37°C)
100 mM NaCl
10 mM MgCl₂
1 mM DTT
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: <25%
Buffer 2: 75%
Buffer 3: 100%
Buffer 4: 75%
Buffer 5: 50%
*star activity 

Storage Buffer

10 mM Tris-HCl (pH 7.5 at 4°C)
50 mM KCl
1 mM dithiothreitol
0.1 mM EDTA
500 µg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions

See Certificate of Analysis Below

Storage Conditions

Store at -20°C
Shipped on dry ice