Dde I

Source

Desulfovibrio desulfuricans

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour in a total reaction volume of 50 μl

Star Activity

No

Heat Inactivation

65°C for 10 minutes

Incubation Temperature

37°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 3:
50 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂
100 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 50%
Buffer 2: 75%
Buffer 3: 100%
Buffer 4: 50%
Buffer 5: 50%

Storage Buffer

20 mM Tris-HCl (pH 8.0 at 4°C)
100 mM NaCl
0.1 mM EDTA
1 mM DTT
0.2% Triton X-100™
50% (v/v) glycerol

Assay Conditions 

See Certificate of Analysis Below

Storage Conditions

Store at -20°C
Shipped on dry ice