Hae III

Source

Haemophilus aegyptius

Reagents Supplied

CMX Buffer 2

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

80°C for 10 minutes

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 2:
10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 2: 100%
*Star activity

Storage Buffer

50 mM Tris‑HCl (pH 7.5 at 4°C)
100 mM KCl
0.5 mM dithiothreitol
500 µg/ml bovine serum albumin
50% (w/v) glycerol

Assay Conditions

10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg lambda DNA
Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl

Storage Conditions

Store at -20°C
Shipped on dry ice