Hinc II

Source

Haemophilus influenzae Rc

Reagents Supplied

CMX Buffer 5

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

No

Incubation Temperature

37°C

Heat Inactivation

65°C for 10 minutes

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 5:
20 mM Tris-HCl (pH 7.0 at 37°C)
5 mM MgCl₂
50 mM KCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 50%
Buffer 2: 50%
Buffer 3: 75%
Buffer 4: >75
Buffer 5: 100%

Storage Buffer

10 mM Tris‑HCl (pH 7.4 at 4°C)
200 mM NaCl
0.1 mM EDTA
1 mM dithiothreitol
500 μg/ml bovine serum albumin
5 μg/ml poly-L-lysine
0.1% Triton X-100™
50% (v/v) glycerol

Assay Conditions

1 mM DTT
100 μg/ml BSA
20 mM Tris‑HCl (pH 7.0 at 37°C)
50 mM KCl
5 mM MgCl₂
1 μg lambda DNA
Incubation is at 37°C for 1 hour in a total reaction volume of 50 μl

Storage Conditions

Store at -20°C
Shipped on dry ice