Hind III

Source

Haemophilus influenzae Rd

Reagents Supplied

CMX Buffer 2

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

65°C for 10 minutes

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 2:
10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: <25%
Buffer 2: 100%
Buffer 3: <25%
Buffer 4: 50%
Buffer 5: <25%
*Star activity 

Storage Buffer

10 mM Tris-HCl (pH 7.4 at 4°C) 
200 mM NaCl
0.5 mM EDTA
1 mM DTT
0.5 mg/ml bovine serum albumin
5 µg/ml Poly-L-lysine
0.1% Triton X-100™
50% (w/v) glycerol

Assay Conditions

50 mM Tris‑HCl (pH 7.5 at 37°C)
10 mM MgCl₂
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg lambda DNA
Reaction volume of 50 µl

Storage Conditions

Store at -20°C
Shipped on dry ice