Mbo ll

Source

Moraxella bovis

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of unmethylated lambda DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

unmethylated lambda DNA

Reaction Buffer

CMX Buffer 3:
50 mM Tris-HCl (pH 7.5 at 37°C)
100mM NaCl
10 mM MgCl₂
1 mM DTT
Note: 100 ug/ml bovine serum albumin is included in the assay but added separately from the reaction buffer

Storage Buffer

10 mM Tris-HCl (pH 7.5 at 4°C)
0.1 mM EDTA
50 mM KCl
1 mM dithiothreitol
0.01% Triton X-100™
100 ug/ml bovine serum albumin
50% glycerol

Assay Conditions

50 mM Tris‑HCl (pH 7.5 at 37°C)
10 mM MgCl₂
100 mM NaCl
1 mM DTT
100 µg/ml BSA
1 µg unmethylated lambda DNA

Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl

Storage Conditions

Store at -20°C
Shipped on dry ice 

Categories

Restriction Endonucleases