Pvu I

Pvu I Source

Proteus vulgaris

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of Lambda/Hind III DNA fragments in 1 hour at 37°C in a total reaction volume of 50 µl.

Star Activity

No

Incubation Temperature

37°C

Heat Inactivation  

10 minutes at 80°C

Assay Unit Substrate

Lambda/Hind III DNA fragments

Reaction Buffer

CMX Buffer 3
50 mM Tris-HCl (pH 7.4 at 37°C)
10 mM MgCl₂
100 mM NaCl₂
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Storage Buffer

50 mM Tris-HCl (pH 7.4 at 4°C)
200 mM KCl 
0.1 mM EDTA
10 mM β-mercaptoethanol
0.2% (v/v) Triton X-100™
200 µg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions

50 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2, 100 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg Lambda/Hind III DNA fragments
Reaction volume 50 µl

Activity in Buffers

Buffer 1: NA
Buffer 2: 75%
Buffer 3: 100%
Buffer 4: 25%
Buffer 5: <25%
Buffer 6: 100%

Storage Conditions

Store at -20°C
Shipped on dry ice 

Categories

Restriction Endonucleases, Pvu I