Rsr II

Rsr II Source

Rhodopseudomonas sphaeroides

Reagents Supplied

CMX Buffer 4

Unit Definition

One unit is the amount of enzyme required to digest 1 µg of lambda DNA with ≥95% efficiency in 1 hour in a total reaction volume of 50 μl

Star Activity

No

Incubation Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 4:
20 mM Tris-Acetate (pH 7.5 at 37ºC)
50 mM Potassium Acetate
10 mM Magnesium Acetate
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 50%
Buffer 2: 75%
Buffer 3: 25%
Buffer 4: 100%
Buffer 5: 50%

Storage Buffer

10 mM Tris‑HCl (pH 7.4 at 4ºC) 
0.1 mM EDTA
250 mM NaCl
1 mM dithiothreitol
200 µg/ml bovine serum albumin
0.15% (v/v) Triton X-100
50% (v/v) glycerol

Assay Conditions

20 mM Tris‑Acetate (pH 7.5 at 37ºC)
50 mM Potassium Acetate
10 mM Magnesium Acetate
1.0 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg lambda DNA
Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl

Notes

1. Lambda DNA contains 3 slow-cleavage sites that do not completely digest after 1 hour incubation.
2. Complete digestion of these sites is achieved by 4 Units of Rsr II with 1 μg of lambda DNA incubated at 37°C for 16 hours.

Store at -20°C
Shipped on dry ice 

Categories

Restriction Endonucleases