Sac II

Sac II Source

Streptomyces achromogenes

Reagents Supplied

CMX Buffer 4

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus 2 DNA in 1 hour at 37°C in a total reaction volume of 50 µl

Star Activity

No

Incubation Temperature

37°C

Heat Inactivation

10 minutes at 65ºC

Assay Unit Substrate

Adenovirus 2 DNA

Reaction Buffer

CMX Buffer 4:
20 mM Tris-acetate (pH 7.5 at 37ºC)
50 mM potassium acetate
10 mM magnesium acetate
1 mM dithiothreitol

Activity in Buffers

Buffer 1: 75%
Buffer 2: 100%
Buffer 3: 75%
Buffer 4: 100%
Buffer 5: 50%

Storage Buffer

50 mM Tris-HCl (pH 7.5 at 4ºC)
0.1 mM EDTA
500 µg/ml bovine serum albumin
1 mM dithiothreitol
0.1% (w/v) Triton X-100™
50% (v/v) glycerol

Assay Conditions

20 mM Tris-acetate (pH 7.5 at 37ºC)
50 mM potassium acetate
10 mM magnesium acetate
1 mM dithiothreitol
1 µg Adenovirus 2 DNA

Storage Conditions

Store at -20°C
Shipped on dry ice 

Categories

Restriction Endonucleases