SaI I

Sal I Source

Streptomyces albus

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of Adenovirus 2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation 

10 minutes at 65°C

Assay Unit Substrate

adenovirus 2 DNA 

Reaction Buffer

CMX Buffer 3:
50 mM Tris-HCl (pH 7.5 at 37ºC)
10 mM MgCl₂
100 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: <25%
Buffer 2: 50%
Buffer 3: 100%
Buffer 4: <25%
Buffer 5: 25%
*Star activity 

Storage Buffer

20 mM potassium phosphate (pH 7.0 at 4ºC) 
200 mM NaCl
1.0 mM EDTA
5 μg/ml Poly L-Lysine
0.0065% sodium azide
1 mM dithiothreitol
500 μg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions

50 mM Tris-HCl (pH 7.5 at 37ºC)
10 mM MgCl₂
100 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg adenovirus 2 DNA 

Storage Conditions

Store at -20°C
Shipped on dry ice 

Categories

Restriction Endonucleases