Sca I

Sca I Source

Streptomyces caespitosus

Reagents Supplied

10X Sca I Reaction Buffer

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation 

10 minutes at 80°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 6:
50 mM Tris-HCl (pH 7.4 at Room Temperature)
6 mM MgCl₂
50 mM NaCl
50 mM of KCl

Activity in Buffers

Buffer 1: <25%
Buffer 2: 50%
Buffer 3: 25%
Buffer 4: <25%
Buffer 5: 25%
Buffer 6: 100%
*Star activity 

Storage Buffer

50 mM Tris-HCl (pH 7.5 at 4ºC)
50 mM KCl
0.1 mM EDTA
1 mM dithiothreitol
500 μg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions

50 mM Tris-HCl (pH 7.4 at Room Temperature)
6 mM MgCl₂
50 mM NaCl
50 mM KCl
1 µg lambda DNA

Storage Conditions

Store at -20°C
Shipped on dry ice 

Categories

Restriction Endonucleases