Sma I

Sma I Source

Serratia marcescens

Reagents Supplied

CMX Buffer 4

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of Lambda/Hind III DNA in 1 hour at 30°C in a total reaction volume of 50 μl

Star Activity

Yes

Incubation Temperature

30°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Adenovirus 2 DNA

Reaction Buffer

CMX Buffer 4:
20 mM Tris-Acetate (pH 7.5 at 37°C)
50 mM potassium acetate
10 mM magnesium acetate
100 µg/ml bovine serum albumin
1 mM dithiothreitol 

Activity in Buffers

Buffer 1: 0%
Buffer 2: 0%
Buffer 3: 0%
Buffer 4: 100%
Buffer 5: 25%
Buffer 6: 25%
*Star activity 

Storage Buffer

20 mM Tris-HCl (pH 7.5 at 4°C)
1 mM EDTA
7 mM β-mercaptoethanol
500 μg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions

20 mM Tris‑acetate (pH 7.5 at 37°C)
50 mM potassium acetate
10 mM magnesium acetate
100 µg/ml bovine serum albumin
1 μg Lambda/Hind III DNA
1 mM dithiothreitol

Storage Conditions

Store at -20°C
Shipped on dry ice 

Categories

Restriction Endonucleases