Spe I

Spe I Source

Sphaerotilus species

Reagents Supplied

CMX Buffer 4

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of pJA650/PSt I DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

pJA650/PSt I DNA

Reaction Buffer

CMX Buffer 4:
20 mM Tris-Acetate (pH 7.5 at 37°C)
10 mM Mg-Acetate
50 mM K-Acetate
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 100%
Buffer 2: 100+%
Buffer 3: 50%
Buffer 4: 100%
Buffer 5: 100%
*Star activity 

Storage Buffer

50 mM Tris-HCl (pH 7.5 at 4°C) 
200 mM NaCl 
0.1 mM EDTA
0.2% (v/v) Triton 
X-100™
1 mM dithiothreitol
500 µg/ml bovine serum albumin 
50% (v/v) glycerol

Assay Conditions

10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
 1 µg Ad-2 DNA

Storage Conditions

Store at -20°C
Shipped on dry ice 

Categories

Restriction Endonucleases