Sph I

Sph I Source

Reagents Supplied

CMX Buffer 6

Unit Definition

Star Activity

Yes

Reaction Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 6

50 mM Tris-HCl (pH 7.4 at Room Temperature)
6 mM MgCl₂
50 mM NaCl
50 mM KCl

Activity in Buffers

Buffer 1: 25%
Buffer 2: 50%
Buffer 3: 75%
Buffer 4: 25%
Buffer 5: 25%
Buffer 6: 100%
*Star activity 

Storage Buffer

50 mM Tris‑HCl (pH 7.5 at 4°C)
1 mM EDTA
100 mM KCl
5 mM β-mercaptoethanol
0.1% Triton X-100™
0.1 mM PMSF
500 µg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions

50 mM Tris‑HCl (pH 7.4 at Room Temperature)
6 mM MgCl₂
50 mM NaCl
50 mM KCl
1 µg lambda DNA

Storage Conditions

Store at -20°C
Shipped on dry ice 

Categories

Restriction Endonucleases