Stu I

Stu I Source

Streptomyces tubercidicus

Reagents Supplied

CMX Buffer 2

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of umethylated lambda DNA in 1 hour at 37°C in a total reaction volume of 50 µl

Star Activity

No

Reaction Temperature

37°C

Heat Inactivation

10 minutes at 65°C 

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 2:
10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 75%
Buffer 2: 100%
Buffer 3: 50%
Buffer 4: 75%
Buffer 5: 75%

Storage Buffer

10 mM Tris‑HCl (pH 7.4 at 4°C) 
50 mM KCl
0.1 mM EDTA
1 mM dithiothreitol
0.5 mg/ml bovine serum albumin
0.1 mM PMSF
50% (v/v) glycerol

Assay Conditions

10 mM Tris-HCl (pH 7.5 at 37°C)
50 mM NaCl
10 mM MgCl₂
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg unmethylated lambda DNA
Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl

Storage Conditions

Store at -20°C
Shipped on dry ice 

Categories

Restriction Endonucleases